Chemical nature of the autoregulating factor d1 in Pseudomonas carboxydoflava

A chromatographically individual fraction with the biological activity of factor d1 was isolated from Pseudomonas carboxydoflava by the techniques of column and thin-layer chromatography. Alkyl hydroxybenzoles were shown to be the active principle of factor d1 inducing the transition of P. carboxydoflava vegetative cells into the hypometabolic or anabiotic state. As was found from the data of PMR, UV and IR spectroscopy and gas chromatography in combination with mass spectrometry, the active principle of factor d1 included alkylresorcins: 5-n-nonadecylresorcin and 5-n-heneicosylresorcin at a ratio of 1:3.